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fpg  (New England Biolabs)


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    Structured Review

    New England Biolabs fpg
    Fpg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 550 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fpg/Fpg/pm42092682-397-23-30
    Average 96 stars, based on 550 article reviews
    fpg - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Hybridization:

    Article Title: Oxalate induces proliferation and mitochondrial metabolism in select clear cell renal cell carcinoma cell lines.
    Article Snippet: Next, cells were permeabilized with Biotium permeabilization buffer (22016, Fremont, CA, USA) with 0.05% Triton-X for 10 min at 37°C. .. The cells were subsequently washed three times with 1X PBS before being exposed to the lesion removal cocktail, FPG (4U, cat#M0240, New England BioLabs (NEB), Ipswich, MA, USA,), T4 PDG (5U, cat#M0308, NEB), UDG (2.5U, cat#M0280, NEB), EndoIV (5U, cat#M0304, NEB), EndoVIII (5U, cat#M0299, NEB), AAG (5U, cat#M0313, NEB) resuspended in 1X ThermPol buffer (cat#B9004S, NEB) + 200 μg/ml BSA (cat#B4287-25G, Sigma Aldrich, St. Louis, MO, USA) for 1 h at 37°C in a hybridization Fluorescent In-Situ Hybridization oven(41). .. Next, the gap-filling mixture of Klenow exo- and digoxigenin-labeled dUTP (cat#11093088910, Sigma Aldrich, St. Louis, MO, USA) in 1X ThermPol buffer + BSA was added to the cells and incubated again at 37°C for 1 h. The cells were washed three times with 1X PBS and blocked with 2% BSA (cat#001-000-162, Jackson ImmunoResearch) in PBS for 30 min. After blocking, the samples were incubated with anti-digoxigenin (1:250; cat#ab420, Abcam) or anti-mouse IgG1 isotype control (1:625; cat#5415, Cell Signaling) primary antibodies for 1 h at RT.

    Article Title: Oxalate induces proliferation and mitochondrial metabolism in select clear cell renal cell carcinoma cell lines
    Article Snippet: Next, cells were permeabilized with Biotium permeabilization buffer (22,016, Fremont, CA, USA) with 0.05% Triton-X for 10 min at 37 °C. .. The cells were subsequently washed three times with 1X PBS before being exposed to the lesion removal cocktail, FPG (4U, cat#M0240, New England BioLabs (NEB), Ipswich, MA, USA,), T4 PDG (5U, cat#M0308, NEB), UDG (2.5U, cat#M0280, NEB), EndoIV (5U, cat#M0304, NEB), EndoVIII (5U, cat#M0299, NEB), AAG (5U, cat#M0313, NEB) resuspended in 1X ThermPol buffer (cat#B9004S, NEB) + 200 μg/ml BSA (cat#B4287-25G, Sigma Aldrich, St. Louis, MO, USA) for 1 h at 37 °C in a hybridization Fluorescent In-Situ Hybridization oven [ ]. .. Next, the gap-filling mixture of Klenow exo- and digoxigenin-labeled dUTP (cat#11,093,088,910, Sigma Aldrich, St. Louis, MO, USA) in 1X ThermPol buffer + BSA was added to the cells and incubated again at 37 °C for 1 h. The cells were washed three times with 1X PBS and blocked with 2% BSA (cat#001–000–162, Jackson ImmunoResearch) in PBS for 30 min. After blocking, the samples were incubated with anti-digoxigenin (1:250; cat#ab420, Abcam) or anti-mouse IgG1 isotype control (1:625; cat#5415, Cell Signaling) primary antibodies for 1 h at RT.

    Article Title: Cigarette smoke induces FASN-dependent fatty acid metabolic rewiring to drive bladder cancer progression.
    Article Snippet: 61 Cigarette smoke promotes bladder tumor growth by enhancing cancer cell survival and 62 proliferation through smoke mediated carcinogens.. FASN, a key enzyme in fatty acid 63 synthesis, is dysregulated in many cancers and correlates with aggressive phenotypes.. 64 In this study, we demonstrate elevated fatty acid levels and FASN specifically in smokers 65 with bladder cancer.

    In Situ:

    Article Title: Oxalate induces proliferation and mitochondrial metabolism in select clear cell renal cell carcinoma cell lines.
    Article Snippet: Next, cells were permeabilized with Biotium permeabilization buffer (22016, Fremont, CA, USA) with 0.05% Triton-X for 10 min at 37°C. .. The cells were subsequently washed three times with 1X PBS before being exposed to the lesion removal cocktail, FPG (4U, cat#M0240, New England BioLabs (NEB), Ipswich, MA, USA,), T4 PDG (5U, cat#M0308, NEB), UDG (2.5U, cat#M0280, NEB), EndoIV (5U, cat#M0304, NEB), EndoVIII (5U, cat#M0299, NEB), AAG (5U, cat#M0313, NEB) resuspended in 1X ThermPol buffer (cat#B9004S, NEB) + 200 μg/ml BSA (cat#B4287-25G, Sigma Aldrich, St. Louis, MO, USA) for 1 h at 37°C in a hybridization Fluorescent In-Situ Hybridization oven(41). .. Next, the gap-filling mixture of Klenow exo- and digoxigenin-labeled dUTP (cat#11093088910, Sigma Aldrich, St. Louis, MO, USA) in 1X ThermPol buffer + BSA was added to the cells and incubated again at 37°C for 1 h. The cells were washed three times with 1X PBS and blocked with 2% BSA (cat#001-000-162, Jackson ImmunoResearch) in PBS for 30 min. After blocking, the samples were incubated with anti-digoxigenin (1:250; cat#ab420, Abcam) or anti-mouse IgG1 isotype control (1:625; cat#5415, Cell Signaling) primary antibodies for 1 h at RT.

    Article Title: Oxalate induces proliferation and mitochondrial metabolism in select clear cell renal cell carcinoma cell lines
    Article Snippet: Next, cells were permeabilized with Biotium permeabilization buffer (22,016, Fremont, CA, USA) with 0.05% Triton-X for 10 min at 37 °C. .. The cells were subsequently washed three times with 1X PBS before being exposed to the lesion removal cocktail, FPG (4U, cat#M0240, New England BioLabs (NEB), Ipswich, MA, USA,), T4 PDG (5U, cat#M0308, NEB), UDG (2.5U, cat#M0280, NEB), EndoIV (5U, cat#M0304, NEB), EndoVIII (5U, cat#M0299, NEB), AAG (5U, cat#M0313, NEB) resuspended in 1X ThermPol buffer (cat#B9004S, NEB) + 200 μg/ml BSA (cat#B4287-25G, Sigma Aldrich, St. Louis, MO, USA) for 1 h at 37 °C in a hybridization Fluorescent In-Situ Hybridization oven [ ]. .. Next, the gap-filling mixture of Klenow exo- and digoxigenin-labeled dUTP (cat#11,093,088,910, Sigma Aldrich, St. Louis, MO, USA) in 1X ThermPol buffer + BSA was added to the cells and incubated again at 37 °C for 1 h. The cells were washed three times with 1X PBS and blocked with 2% BSA (cat#001–000–162, Jackson ImmunoResearch) in PBS for 30 min. After blocking, the samples were incubated with anti-digoxigenin (1:250; cat#ab420, Abcam) or anti-mouse IgG1 isotype control (1:625; cat#5415, Cell Signaling) primary antibodies for 1 h at RT.

    Article Title: Cigarette smoke induces FASN-dependent fatty acid metabolic rewiring to drive bladder cancer progression.
    Article Snippet: 61 Cigarette smoke promotes bladder tumor growth by enhancing cancer cell survival and 62 proliferation through smoke mediated carcinogens.. FASN, a key enzyme in fatty acid 63 synthesis, is dysregulated in many cancers and correlates with aggressive phenotypes.. 64 In this study, we demonstrate elevated fatty acid levels and FASN specifically in smokers 65 with bladder cancer.

    other:


    Incubation:

    Article Title: Parkinson’s disease linked LRRK2 G2019S drives oxidative nuclear DNA damage and PARP1 hyperactive signaling
    Article Snippet: Cells were permeabilized using permeabilization buffer (Biotium, Cat. # 22016) with 0.05% Triton X-100 (Sigma Aldrich, X100-5ml) for 10 minutes at room temperature, then washed twice with PBS. .. For lesion removal, cells were washed once with water, and then incubated with 100 μL of a lesion removal mix consisting of 10 μL Thermo Pol Buffer (New England Biolabs, B9004), 500 μM NAD + (New England Biolabs, B9007), 200 μg/mL BSA (Sigma Aldrich, A2058), 4 U FPG (NEB, M0240), 5 U EndoIV (New England Biolabs M0304), and 5 U EndoVIII (New England Biolabs, M0299) for 1 h at 37°C. .. Cells were then incubated with 100 μL of a gap-filling mix consisting of 1 μL Klenow exo− (ThermoFisher, EP0422), 0.1 μL Digoxigenin dUTP (Sigma Aldrich, 11093088910), 200 μg/mL BSA, and 10 μL Thermo Pol Buffer (New England Biolabs, B9004) for 1 h at 37°C.

    Control:

    Article Title: Catalytic and Structural Insights into Neil3-Dependent Unhooking of Endogenous Abasic DNA Crosslink
    Article Snippet: .. Control enzymes with well-characterised cleavage activities, including Nth and Fpg (New England Biolabs) , were used for comparison. ..

    Comparison:

    Article Title: Catalytic and Structural Insights into Neil3-Dependent Unhooking of Endogenous Abasic DNA Crosslink
    Article Snippet: .. Control enzymes with well-characterised cleavage activities, including Nth and Fpg (New England Biolabs) , were used for comparison. ..



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